anti chick β1 subunit monoclonal antibody v2e9  (Developmental Studies Hybridoma Bank)

Journal: The Journal of Neuroscience

Article Title: Comparison of Neurite Outgrowth Induced by Intact and Injured Sciatic Nerves: A Confocal and Functional Analysis

doi: 10.1523/JNEUROSCI.18-01-00328.1998

Figure Lengend Snippet: Effect of anti-β1-integrin antibodies on neurite outgrowth of sensory neurons grown for 48 hr in Terasaki dishes on laminin substrata

Article Snippet: The monoclonal antibodies (mAbs) directed against the chick β1-integrin subunit, JG22 (David I. Gottlieb; Greve and Gottlieb, 1982 ), V2E9 (Alan F. Horwitz; Hayashi et al., 1990 ), and against chicken N-CAM, 5e (Urs Rutishauser), were obtained from the Developmental Studies Hybridoma Bank maintained by the Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, and the Department of Biological Sciences, University of Iowa, Iowa City, IA 52242, under contract N01-HD-6-2915 from the National Institute of Child Health and Human Development.

Techniques: Inhibition

Journal: The Journal of Neuroscience

Article Title: Comparison of Neurite Outgrowth Induced by Intact and Injured Sciatic Nerves: A Confocal and Functional Analysis

doi: 10.1523/JNEUROSCI.18-01-00328.1998

Figure Lengend Snippet: Inhibition of neurite outgrowth of E13 chick sensory neurons by two different antibodies, JG22 and V2E9, directed against chick β1-integrin subunit, on each type of nerve substratum. The concentrations used were 25 μg Ig/ml for both antibodies. The blocking ability of each antibody was controlled with the same neuronal preparations grown on laminin-1 or laminin-2, and the corresponding results are shown in Table ​Table1.1. A, Quantification of the number of neurons extending neurites after 2 d of culture. Values represent mean and SEM of the percentage of growing neurons for five different experiments. B, Quantification of neurite length. Values represent the mean and SEM of total neuritic length elaborated per neuron, measured for at least 120 neurons.*p < 0.05. Significance was analyzed using the Student’s t test.

Article Snippet: The monoclonal antibodies (mAbs) directed against the chick β1-integrin subunit, JG22 (David I. Gottlieb; Greve and Gottlieb, 1982 ), V2E9 (Alan F. Horwitz; Hayashi et al., 1990 ), and against chicken N-CAM, 5e (Urs Rutishauser), were obtained from the Developmental Studies Hybridoma Bank maintained by the Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, and the Department of Biological Sciences, University of Iowa, Iowa City, IA 52242, under contract N01-HD-6-2915 from the National Institute of Child Health and Human Development.

Techniques: Inhibition, Blocking Assay

Journal: The Journal of Neuroscience

Article Title: Comparison of Neurite Outgrowth Induced by Intact and Injured Sciatic Nerves: A Confocal and Functional Analysis

doi: 10.1523/JNEUROSCI.18-01-00328.1998

Figure Lengend Snippet: Effects of anti-β1-integrin antibodies (JG22) and of anti-ECM molecule antibodies on the number of primary neurites elaborated per sensory neuron, cultivated on sections of normal and degenerated sciatic nerve

Article Snippet: The monoclonal antibodies (mAbs) directed against the chick β1-integrin subunit, JG22 (David I. Gottlieb; Greve and Gottlieb, 1982 ), V2E9 (Alan F. Horwitz; Hayashi et al., 1990 ), and against chicken N-CAM, 5e (Urs Rutishauser), were obtained from the Developmental Studies Hybridoma Bank maintained by the Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, and the Department of Biological Sciences, University of Iowa, Iowa City, IA 52242, under contract N01-HD-6-2915 from the National Institute of Child Health and Human Development.

Techniques: Inhibition